• The Jason T Maynes Lab

    Improving Patient Outcomes by Intersecting Clinical Care,

    Disease Pathophysiology, and Biophysics

  • WHAT WE DO

    Our Mission is to Link Patient Disease with Molecular Mechanism and Generate New Therapeutic Avenues in the Pediatric Population

    Focal Adhesion Imaging in iPSC-cardiomyocytes

    Heart Failure, Cardiac Dysfunction and Stem Cell-Derived Tissue

    Integrin-linked Kinase and New Therapies for Heart Failure

    Integrin-linked kinase (ILK) is a pseudo-kinase (i.e. kinase fold without protein phosphorylation activity) that scaffolds focal adhesion proteins in many cell types, including cardiomyocytes. We have illustrated how ILK can also improve cardiac inotropy through the regulation of cellular calcium currents. Using iPSC-cardiomyocyte models, we have shown how ILK interacts with the calcium pump SERCA-2a and how upregulation of ILK can prevent cancer chemotherapy-induced cardiac dysfunction and reduce arrhythmias. We continue to determine key ILK protein:protein interactions that affect cardiomyocyte function and develop new therapeutics for heart failure based on ILK.

     

    Mitochondrial and Peroxosome Imaging

    Mitochondrial Function, Cellular Energy and Metabolomics

    Mitochondrial Form and Function in Disease States

    Mitochondrial function is increasingly being discovered to be important in disease processes. Using biophysical methods, we study how both the function of the mitochondrial and the shape of the organelle is altered by disease and pharmacology. Mitochondrial shape (covered by fusion and fission) is a newly discovered mechanism by which mitochondria repair their protein and DNA components, in particular under the constantly oxidative environment of the mitochondria.

    Examples of projects ongoing in this area include:

    • the biochemical and biophysical characterization of proteins involved in mitochondrial fusion and fission
    • metabolomics of cardiac disease, including heart failure and pulmonary hypertension
    • how anesthetic agents alter cellular metabolism and affect patient outcome

     

    Drug Effects on Ion Currents in iPSC-cardiomyocytes

    Drug Design and Therapy Discovery

    Advancements in Measuring Cardiac and Cardiomyocyte Function

    In order to develop new cardiac therapies and predict pre-clinical cardiac drug toxicity, the effect of pharmaceuticals on cardiac and cardiomyocyte function needs to be measured in ways that translate accurately to patient response. We develop new methodologies that allow for more accurate quantification of drug effect, cardiac and non-cardiac. This includes the development of iPSC-derived tissue drug testing, measurement of cardiomyocyte arrhythmia and contractility, and image analysis algorithms.

     

    Examples of projects ongoing in this area include:

    • new therapies for viral infections
    • novel therapies for heart failure based on calcium cycling
    • platform technologies for measuring cardiomyocyte contractility and gap junction activity

     

     

    Drug Effects on Ion Currents in iPSC-cardiomyocytes

    The Cellular Effects of Anesthetics

    Determining How Anesthetic Agents Affect Cellular Processes

    One of the largest issues facing pediatric anesthesia and surgery is the potential adverse effects of anesthetics on the developing brain. We investigate how pharmaceuticals used in a common anesthesia - inhalational and IV anesthetics, opiates, benzodiazepines - affect various cellular processes including protein function and metabolism.

     

    Examples of projects ongoing in this area include:

    • the effects of anesthetics on protein folding
    • how autistic patients are affected by anesthesia
    • how anesthetic agents affect gene transcriptional regulation

     

     

     

  • WHO WE ARE

    Current Lab Members

    Jason T Maynes, PhD/MD

    aka Gru, Chief Man, Boss man

    Jason T Maynes, PhD/MD is the Director of Research for Anesthesia and Pain Medicine and a Staff Anesthesiologist at The Hospital for Sick Children. He has research training in biophysics from the University of Alberta, Los Alamos National Weapons Lab and Washington University.

     

    Favorite Food or Drink: Scotch

    Favorite Quote: " Science is the belief in the ignorance of the experts." - Richard Feynman

    Ramesh Vanama, PhD

    Lab Manager

    aka The Man

    Indian, speaks Telugu, not as mean as the picture looks.

     

     

    Yanan Tang, PhD

    Research Associate

    I received my Ph.D degree in 2013 from University of Alberta under the supervision of Dr. Liang Li. My research was focused on the development of mass spectrometric methodologies with high throughput and high sensitivity to facilitate the analysis of biological molecules, such as peptides, proteins and metabolites, in complex sample matrices. I’m especially interested in applying mass spectrometry based “omics” strategy to clinical sample analysis in order to unveil the molecular mechanisms of diseases and find biomarkers.

     

     

    Michael Tropak, PhD

    Research Associate

    Development of assays to search through chemical space for compounds targeting disease relevant proteins. Biochemistry and microscopic imagery of CRISPR modified cell lines to understand the molecular basis of the pathology associated with disease relevant genes. Procurement of resources and woodworker.

     

     

    Julia Plakhotnik

    Graduate Student

    Hi, I'm Julia! I am currently a PhD student in the Department of Biochemistry at UofT, and previously graduated co-op Biochemistry at the University of Waterloo. I study proteins that relay tension in the heart. I pretend to rule over the elements, but really, I just mix colourful liquids.

     

     

    Manpreet Malhi

    Graduate Student

    I am a PhD candidate in the Maynes laboratory focusing on research pertaining to drug discovery and development. More specifically, I am investigating novel small molecular inhibitors of respiratory syncytial virus. Prior to joining the laboratory, I obtained a Bachelor of Science in Biochemistry and a Master’s of Biomedical Technology from the University of Calgary.

     

     

    Doorsa Tarazi

    Graduate Student

    I did my undergrad in biochemistry at McGill and decided to move back to Toronto because of the wonderful performance of our sports teams. I am currently investigating the effects of anesthetics and analgesics on cancer metabolism, methylation, and growth. I’m mesmerized by dry ice. If you have some by your desk, be assured, I will be popping by to ‘borrow’ a piece or two.

     

     

    Olga Chernysh, PhD

    Technician

    Olga was born on Earth. She continues to live there. She enjoys eating and sleeping. More to follow.

     

     

    Kaley Hogarth

    Technician

    Hi, I’m Kaley! I am currently a laboratory technician in the JTM lab. Prior to starting here, I completed an undergraduate degree in Molecular Biology & Genetics and Biomedical Sciences and a master’s degree in Pharmacology & Toxicology, both from the University of Guelph. When I’m not working on my power swing, I find time to study the effects of various anesthetics on mitochondrial function. Still working on hitting for the Krebs cycle!

     

     

    Carolyne Pehora

    Research Nurse

    I graduated from the University of Toronto with a Bachelor of Science in Nursing Degree and obtained my Master of Nursing Degree from Athabasca University. By day, I help coordinate research studies in the Department of Anesthesia and Pain Medicine and am responsible for various nursing/research-related tasks. By night I am a part time fitness/Zumba instructor and also enjoy tap dancing, playing the piano and travelling the world.

     

     

    David Pompili

    Research Student

    I completed my undergrad at the University of Toronto in the departments of Human Biology and Cell and Systems Biology. I am currently looking at the effects of focal adhesion proteins on cancer cell invasion, motility, and proliferation. I am an avid sports lover and our lab’s resident Rubik’s Cube grandmaster. Be careful where you leave your cubes as I may not be able to resist the sudden urge to solve them.

     

     

    Megan Banting

    Research Student

    Hi I'm Megan! I recently graduated from the University of Western Ontario with a BSc in Physiology. I am currently volunteering, working on a bedside assay to screen for milrinone serum levels. There are no small projects just small scientists.

     

    *Calculator and pen are pocket size

  • Equipment Bookings

    For booking for lab equipment specific to the Maynes Lab, or change to HSC equipment page

  • Past Lab Members

    Most are missed.....

    Fellows (current location/position)

    • Daniel Stocki: Tel-Aviv Sourasky Medical Center (Tel-Aviv, Israel)
    • Dean Bunbury: Middlemore Hospital (Auckland City, New Zealand)
    • Sindu Balakrishnan: Hamad General Hospital (Doha, Qatar)
    • Matthew Coghlan: Our Lady's Children's Hospital, Crumlin (Dublin, Ireland)
    • Diana Raj: Gartnaval Royal Hospital (Glascow, Scotland)
    • Vannessa Chin: Hospital for Sick Children (Toronto, Canada)
    • Grant Stewart: Greater Ormond Street Hospital (London, England)

    Students (current location/position)

    • Lawrence Wengle: University College Cork (Cork, Ireland)
    • Megan Banting:

     

  • Contact Us

    Shine on you crazy diamond.

    Email

    Address

     

    Department of Anesthesia and Pain Medicine

    Hospital for Sick Children

    Toronto, Ontario, Canada

    M5G 1X8

     

    Phone

     

    416.813.5934

     

    Email

    Email

     

    jason.maynes@sickkids.ca